ywpJ [2019-02-22 11:43:46]

putative [SW|HAD superfamily] phosphatase

locus: BSU36290

pI: 4.88

mw: 31.80 kDa

protein length: 285 aa

gene length: 858 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,738,343 → 3,739,200

The protein

Protein family

• [SW|HAD superfamily]
• Cof family (according to Swiss-Prot)

Modification

• phosphorylated on Arg-258 [Pubmed|22517742]

Structure

• [PDB|1NRW]

Expression and Regulation

Operons

• genes: [gene|6F5831A480B72D17B7B3F0BF7AF147603B65CA49|glcR]-[gene|AF9523D3AA3C032B70175BCFFED34E9A42ED01AF|phoC]
  description: [Pubmed|30782637,22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|30782637], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|6F5831A480B72D17B7B3F0BF7AF147603B65CA49|GlcR]: repression, [pubmed|30782637], in [regulon|6F5831A480B72D17B7B3F0BF7AF147603B65CA49|GlcR regulon]

  regulation

  • induced by phosphosugar stress [Pubmed|30782637]

Biological materials

Mutant

• MGNA-A531 (ywpJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/531 NBRP B. subtilis, Japan]
• BKE36290 (Δ[gene|AF9523D3AA3C032B70175BCFFED34E9A42ED01AF|phoC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCGCAATTAATTTCACGT, downstream forward: _UP4_TAAGTATATGTGCTGCCACA
• BKK36290 (Δ[gene|AF9523D3AA3C032B70175BCFFED34E9A42ED01AF|phoC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCGCAATTAATTTCACGT, downstream forward: _UP4_TAAGTATATGTGCTGCCACA

References

• 22383849,22517742